MULTIPLEX IMMUNOFLUORESCENCE PROFILING OF TUMOR BIOPSIES USING ULTRAPLEX MXIF TECHNOLOGY
An unmet need in tissue diagnostics is the ability to simultaneously detect three or more markers on a single tissue specimen, i.e. multiplexing. Unlike other immunoassays, i.e. flow cytometry, ELISA and bead assays, which solely quantify the biomarker in a sample, a multiplex immunoassay quantifies the biomarker, identifies the location of the cell in the tissue and identifies the spatial relationship of the biomarkers with respect to each other. Being able to identify the contextual relationships of immune cell phenotypes and other biomarkers in a tumor is believed to be critical to understanding the state of the immune system before and after immunotherapy. Multiplex immunostaining can also be described as image cytometry or flow cytometry in situ.